Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the MMP‐2 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. MMP‐2 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the TNF‐α and IL‐6 levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. (A) represents subsequent time points for TNF‐α analysis, and (B) represents subsequent time points for IL‐6 analysis. TNF‐α and IL‐6 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IL‐6: interleukin‐6; MMP‐2, matrix metalloproteinase‐2; P 4 , progesterone; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the VEGF‐A levels in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M)+ ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser + E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser+ E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. VEGF‐A levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; P 4 , progesterone; VEGF‐A, vascular endothelial growth factor‐A.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification

Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the IGFBP‐1 secretions in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser+ E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser + E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. IGFBP‐1 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IGFBP‐1, insulin‐like growth factor‐binding protein‐1; P 4 , progesterone.

Article Snippet: To evaluate the remodeling effect of Er‐YAG laser on endometrial tissue, matrix metalloproteinase‐2 (MMP‐2) levels in conditioned media were measured by an enzyme‐linked immunosorbent assay (ELISA) (Elabscience; E‐EL‐H1445, Houston, TX, USA), which has a sensitivity of 0.47 ng/mL and no reported cross‐reactivity or interference [ ].

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification, Binding Assay

Journal: Bioactive Materials

Article Title: Three-dimensional bioprinted hiHeps hepatorganoids with enhanced hepatic functions for the treatment of liver failure and promotion of liver regeneration

doi: 10.1016/j.bioactmat.2025.12.024

Figure Lengend Snippet: 3DP-HHO enhances liver regeneration via upregulation of MMPs and HGF signaling and improves survival in PHLF. A. qRT-PCR analysis of MMP2 and MMP9 in 2D-Cultured hiHeps and 3DP-HHO (n = 3 per group). B. ELISA analysis of culture supernatants in 2D-Cultured hiHeps and 3DP-HHO (n = 4 per group). C. qRT-PCR analysis of HGF in 2D-Cultured hiHeps and 3DP-HHO (n = 4 per group). D. ELISA analysis of serum samples at 0-, 24- and 48-h post-surgery in each experimental group in PHx model (n = 3–5 per group). E. Representative images of cell migration using Transwell assays in 2D-Cultured hiHeps and 3DP-HHO. Scale bars: 250 μm. F. Representative immunofluorescence images of Human Specific GAPDH (red), ALB (green), and DAPI (blue) of liver sections at day 0 and day 7 post- 3DP-HHO implantation or sham surgery. Scale bars: 100 μm. G. Representative immunofluorescence images of Cyclin D1 (red), and DAPI (blue) of liver sections at 24- and 48-h post-surgery in each experimental group in partial hepatectomy model. Scale bars: 50 μm. H. Quantitative of cell migration using Transwell assays in 2D-Cultured hiHeps and 3DP-HHO (n = 3 per group). I. Quantification of Cyclin D1-positive nuclei of liver sections at 24- and 48-h post-surgery in each experimental group in PHx model (n = 5 per group). J. Survival analysis of 85 % PHx with or without 3DP-HHO implantation. K. Survival analysis of 90 % PHx with or without 3DP-HHO implantation. L. Schematic illustration showing the proposed mechanism by which 3DP-HHO promote liver regeneration through stimulation of hepatocyte migration and HGF/MMP-mediated extracellular remodeling.

Article Snippet: Protein concentrations in culture supernatants or serum samples were measured using ELISA kits (Human ALB ELISA Kit, RK00157, ABclonal, China; Human MMP-2 ELISA Kit, RK00309, ABclonal, China; Human MMP-9 ELISA Kit, RK00217, ABclonal, China; Mouse HGF ELISA Kit, RK00371, ABclonal, China).

Techniques: Quantitative RT-PCR, Cell Culture, Enzyme-linked Immunosorbent Assay, Migration, Immunofluorescence